Nanoporous tent wetting fluorescence inconclusive
Due to blog troubles yesterday, we have chosen to upload this post as a .pdf: Nanoporoustentwettingfluorescenceinconclusive
Similar Posts
Gold Separation Wafer Statistics
Here is the analysis of the wafers used in the pressurized gold separations: SC 199 Stat Result Mean Equiv Diameter 17.84 nm Median Diameter 18.30 nm Porosity 2.2% Weighted Mean Diameter 20.81 nm 90% Diameter 26.64 nm Roundness .782 Separates 10 nm from 15 nm w225 Stat Result Mean Equiv Diameter 18.33 nm…
hToC Modular Approach
Background: The human Tendon-on-Chip (hToC) spans collaboration between the Center for Musculoskeletal Research (CMSR) at the UR and the McGrath lab to develop an in vitro platform to study inflammation, fibrosis, and cell-cycle regulation in fibrovascular tendon healing. Interaction between the blood-tendon-barrier (BTB) is fundamental to the investigation of this pathology as part of the…
Staining nitrocellulose and PDMS array
I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was too dark, as seen by the image below. Harold Smith gave me some Ponceau S…
Transwell Edge Effects – Part 2
A while ago, I posted some images of cells grown on commercial polyester (PET) transwells. Here’s the post. I found that there was an annulus of dead cells around the edge of the transwell when cells were grown on the bottom of the membrane. I contacted Corning and they said that these dead cells were…
Beadazzled: Laying the Foundation of the μSiM-DX Fouling-based Sensor
Starting in December of 2019, we began capturing fluorescent beads on microslit silicon nanomembranes as an illustrative representation of particle capture within our microfluidic devices. From then until now we have successfully used this method within gasket made TFAC devices and uSiM ALine devices using a range of micropore sizes/shapes and bead sizes. These experiments…
Pore-by-pore air flow calculations
Molar flow thought pores with different diameters under 1 psi. First, I calculated molar flow based on porosity histograms extracted from TEM images. And then compared it with the actual experimental data. Here are few examples of this. molar%20flow.ppt
There is a method for direct determination of mass within the optical path which may be valuable here, and produces interference fringes similar to what i can already see in your images:
http://en.wikipedia.org/wiki/Classical_interference_microscopy
When Greg is in Nottingham later in the year we can make a pilgrimage to a colleagues scope in Glasgow and quantify this directly, if you haven’t cracked it already by then 🙂