(Journal Club) Measuring the Zetapotential and Protein Translocation Behavior of Single-pore SiN

This paper (SiN paper) by Ulrich Rant et. Al. details several experiments conducted with a single pore drilled through a SiN membrane via TEM. They measure the relationship of pH vs. zetapotential for both a model protein (avidin) and their membrane, and then see how protein moves across the membrane at different pH’s.

zetapH

Screen Shot 2014-06-24 at 8.48.13 AM

Screen Shot 2014-06-24 at 8.47.19 AM

Translocation is related to the difference in surface charge between the membrane and protein, and diffusion plays an interesting role.

Similar Posts

Leave a Reply