Makerware 3d print Preview
One neat feature of the Makerware software is to visualize the printed layers before actually printing them. You can catch a lot of errors with this feature; I highly recommend it.
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Culture divider insert assembly and test
Introduction We are designing a cell culture chamber insert to allow side-by-side co-culture of cells and ultimately triculture in the µSiM devices. Materials and Methods ALine devices assembly according to the “ALine Modular Device Assembly” instructions. 2-slot NPN membranes applied (NPSN100-2L, nanoporous silicon nitride 100 nm thick, 2 windows). Straight tweezers and chip tweezers are…
Monocyte Isolation via CD14+ Selection (Protocol)
Please see the attached .PDF file for a full monocyte isolation protocol. This is for isolating human monocytes from whole blood. Monocyte-Isolation-Protocol
Rotation Project Complete: Dialysis of cytochrome c across a commercial membrane
Dan Steiner Rotation Summary Abstract The purpose of this rotation project was to develop and test a clampable system for eventual use in scalable nanomembrane dialysis systems. The nanomembrane dialysis system will be miniaturized into a compact, wearable hemodialysis device that will provide more effective human dialysis treatment (Johnson et al. 2013). For initial tests, a…
Wetting of Nanocavities using Isobaric Cooling
Summary and Instructional video The simplest way to get something wet is to allow water to condense on the surface of the something. In our case, we have a number of nanocavities (~ 0.1 femtoliters) with porous tents over them. The easiest thing to do is simply chill the substrates down to allow condensation to form….
Nanomembranes+Microfluidic PETLs Integration
Our collaborator Fernando has made a really nifty way to make microfluidics work with lamination pouches. These flat pouches can be sealed together using a heat activated glue, which can be done consistently using a 15 for a 100-pack of 8.5″x12″ pouches + $40 laminator Put multiple materials and multiple chips in the same microfluidic package….
Schematic of Long Term Clearance Test Setup.
This is the setup used for the long term clearance testing. The fraction collector can be set to advance to the next collection tube at set intervals from 1 min to 99 min. I am currently conducting an evaporation test with both PBS and 100% serum. I weighed six collection tubes, two were filled with…