We still need help with NanoDays
http://doodle.com/
There are two time slots:
10:45am-2pm
1:45-4pm
Please wear your I Love Nano shirts from last year. I have a couple more size Medium and one size Small.
The Rochester Museum & Science Center is on the corner of Goodman and East Ave.
Our new diffusion chamber is finally here. Take a look at the photos first – in our next group meeting you can see the real thing. Today I will test its usability and reliability. Please keep in mind this is only the prototype, there must be upside space for improvement.
Using a 6″ wafer, we start with 50 nm of RV SiN, then deposit 40 nm a-Si followed by 20 nm of SiO2. RTP of 1050 C, 100 C/s, 60s. JP patterned the wafer to make standard SEPCON chips. The pores were transferred to the SiN using RIE. The RIE etches faster at the perimeter…
I finished the first experiment to look at rhodamine transport across commercial transwell membranes. I was mainly interested if there would be enough transport into the basolateral chamber to measure the rhodamine with the spec. Experimental details: both chambers in PBS and at room temperature, spinning on the rotomix, 0.5mM initial rhodamine in donor; sample…
We’ve recently put together our own protein ladder, which contains myosin heavy chain, beta-galactosidase, phosphorylase b, IgG, albumin, and cytochrome c. This is my first attempt running this ladder on w398, one of the new pinhole free membranes. From the TEM pics, it seems that w398 has pores all around the 50nm range, which is…
I found this article about a macro-microfluidic interconnect that I want to try out at some point. We might even be able to seal tube-block interface with PDMS for a tighter seal. http://blogs.rsc.org/chipsandtips/2006/11/13/a-simple-interface-between-a-pdms-microfluidic-device-and-a-syringe/ A simple interface between a PDMS microfluidic device and a syringe 13 Nov 2006 By Francesca Burgoyne. Jeonggi Seo and Meng…
SepCon Experiment (Carrie): Does it work in a SepCon format? Use “Protein A Beads and IgG Binding and Filtering (with SepCon Format)” 9/29/09 Make Buffer B at pH 3 9/30/09 1) Rinse off ethanol 2) Add IgG Take absorbance reading of 1 aliquot of IgG (2×10μl samples) [D7= 0.565abs, D8=0.63abs] Transfer beads to 2ml centrifuge…
