Endothelial Vacuoles Induced by Flow
In working on a long over due paper by Barrett on endothelial response to high permeability material …
Key figs …
And one post from Nakul.
I came across this highly relevant paper
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SU-8 Surface Modification
Last summer, I attempted to modify the surface of SU-8. The purpose of the experiment was to covalently bond poly(ethylene glycol) (PEG) to SU-8, which would be useful when the SU-8 is used as a scaffold on the membranes. The method of surface modification was derived from the attached article, which can be found here. …
Implantable Kidney
DailyHemo Report of Dr. Fissell’s Implantable Kidney Development Sometimes the link above “Implantable Kidney” doesn’t seem to work. http://www.dailyhemo.org/implantable-kidney
PLA Plastic Acidifies DMEM + 10% FBS
I have been looking into PLA (Poly Lactic Acid) plastic for use in our microfluidics. PLA is a commonly used material in 3-d printers, so it would be great if we could print out our microchannels for cell-culture devices. To that end, I wanted to see if the PLA plastic would react with our…
Ultrathin Polymer Membranes with patterned, micrometric pores for organs-on-chip
ByJimPaper for Friday’s Journal Club … Watch this space for a summary and analysis. Pensabene(Hofmeister)ACSAMI16 Synopsis 2um(?) pores in 100 nm thick PLLA. Very, very low porosity. Fabricated with a PVA microneedle mold produced with femto second laser. Microfluidic device integration Claims about cell (HUVEC) viability and growth are poorly supported. Cells are essentially on…
New Alt. Membrane Release Recipe (Nitride RIE)
I have lost many chips during my MgF2 or Au or Pt process due to yield problems with my RIE. I have wondered if the problem was due to power spikes. I have observed that my most poorly yielding etch runs usually have uneven power or power spikes during the run. Increasing window size (active area)…
Long term discoloration II
There have been differences between the previous long term discoloration studies carried out by myself and Barrett. Couple of reasons speculated for the differences were: 1) Wafer variation (w683 vs w704); w683: has no visible pores, is more amorphous in character, and has many pinholes; w704: 200um series. 2) Silanization treatment (UVO chamber PDMS contamination;…