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Makerbot Replicator 2x Open for ABS prints
Recently, Jim has purchased a Makerbot 2x printer for our group. I’ve spent the last few weeks printing and debugging. It has the capability to print many different types of material, along with 2 separate nozzles. I have been able to get many fine prints done with ABS plastic, and I’ve put together a basic…
Pore Processing Results
I have been using MatLab to analyze the pores in the SC wafers. Here are the results from my analyses: To take a first look, I simply plotted four characteristics: average pore size, porosity, cut-off pore size, and mean roundness against the wafer number. Notice that the blue dots are the b wafers. Excel would…
Innocent Until Proven Guilty: Evaluating Pore Patency Across 0.5 μm Microporous Membranes for use in μSiM-CA Bacterial Assays
When we first began developing a standardized protocol for μSiM-CA bacterial assays (described here), bad assay performance led some to believe our issues were due to availability of pores within the membranes inside of the μSiMs. At the same time, I developed a visualization protocol to view bacterial transmission across the membrane (described here). While…
Attempt at movable cutoffs
In the next paper that I’d like to publish, I will compare protein separations for two membranes with different pore distributions to a single membrane at two salt concentrations (basically a redo of this post). My most recent trial with this had less than stellar results. I chose two wafers for movable cutoffs – SC…
Alexander Shestopalov’s Group Update1: Silane with PEG4 and PEG8 Samples
ByXunzhiIn this post, I’m going to discuss the results of the different PEG molecular on silane samples. We used same condition to make 4 chips for each sample. And we treat them with BSA, IgG, and PBS to rest the adsorption. We are using the SEM, contact angle and XPS as the testing method. The way we…
New Wafers
A few of the newest SC wafers came back with intact samples without any pinholes. First here is a list of the experiments that we want to do in our lab with intact/pinhole free membranes 1. Protein separations – Requires >10 samples per wafer (preferably even more) for replicates of separations in both pressure and…
