Movable Cutoffs in Diffusion

In my last discoloration experiment, I tested buffers with different pKa’s (MES, HEPES, and Tris). I found that only 10 mM HEPES in HBSS caused discoloration, and furthermore, that the HBSS component of this solution was the culprit (post). Fortunately, HBSS is a rather simple solution; it only consists of KCl, NaCl, Glucose, KH2PO4, Na2HPO4…

From the past experiments we know that it is not easy to form pores in thicker silicon layer. It is mainly because that higher energy is required to form open holes in a thicker silicon film. Dave showed us less but bigger pores were formed in a 50nm thick silicon film after annealing. According to…

This post presents cell images from the first successful co-culture experiment.  I seeded P8 bEnd3 cells alone, P13 NG108-15 cells alone and both cells on PET and SC500 pnc-Si transwells.  For this experiment, I seeded 50,000 bEnd3/cm2 on the apical side and 50000 NG108-15/cm2 on the basolateral side of the transwell.  I tracked TEER (future…

Summary After success with the development and execution of the μSiM-CA (Canalicular Array; see Masters et al., Nanomedicine: Nanotechnology, Biology, and Medicine 2019), Jim wanted to develop a platform to answer some more biophysics focused questions. In order to facilitate these studies, the platform would ideally be configured to allow for live imaging of staph…

[Background read – https://trace-bmps.org/blog/theory/2015/01/28/why-teer-values-are-multiplied-by-membrane-area-dc-version] I am putting the excerpt of this old post from Jim in this post.   Tom helped me in gathering this data. In order to prove my point that electrical resistance is very sensitive to the membrane geometry, I decided to perform some proof-of-concept experiments with regular transwells. I used 3…