HUVEC Growth and associated pnc-Si discoloration

This is a follow up to my previous post, “Burst Pressure Probability II”. I burst 111, 30nm thick single window pnc-SI TEM chips of ten different window sizes, to measure the burst pressure of chips with varying dimensions. Although area is a major determining factor in the pressure at which the windows break, two different windows of…

In my last post on HUVEC intercellular junctions, I just showed cadherin staining.  I repeated this protocol but also tried immunofluorescence for ZO-1, the tight junction protein. These are HUVEC P7, 6 days post-seeding @ 50,000 cells/cm2.  I looked at glass coverslips, pnc-Si and commercial PET transwells.  Since background fluorescence was so high in my…

I have uploaded the TEOS process outline: TEOS is tetra-ethyl-ortho-silicate, or equivalently tetra-ethoxy-silane. TEOS slowly hydrolyzes into silicon dioxide and ethanol when in contact with ambient moisture. IMPROVEMENTS: -This process prevents us from having to expose the front-side of the silicon during processing. Concurrently, this eliminates the need for aggressive cleaning of the front surface…

The human IgG did not seem to diffuse through the membrane the first time we conducted a diffusion experiment. Barrett believed that the lack of diffusion was most likely due to protein aggregates blocking the pores. So, the human IgG was filtered, and this post is the result of a second round of diffusion experiments….

Because the Barcikowski lab intends to use the nanoparticles they sent us, relatively high yields are necessary for any step that cleans them up. This is unfortunate, because in every single separation that we’ve done (with one exception mentioned further on) we never see yields higher than about 5%. We’d thought that the problem was…