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To Pulldown or Not to Pulldown, That is the Question: Testing the Utility of a Pulldown in µSiM-CA Bacteria Assays
The idea of adding a pulldown modification to our bacteria assay protocol came about after we started to believe that pore discovery was our rate limiting step of the transmission process, discussed here. Initially, it seemed to benefit our assay as it bumped our performance up significantly. Upon closer inspection we realized that we had…
Karl's Air Permeability and Burst Pressure Raw Data
In this week’s meeting there was a request to have Maryna and I make our BP and AP data available online. While Maryna is at the Gordon Conference I thought I would upload what I have. The attached spreadsheet contains two tabs, one for AP and BP. Wafer number and position of each test sample…
Resistance of coated membrane cells
ByJimSamples are from wafers #316 and 318. This is the first time for wafer scale deposition. Processing conditions:1) chamber base pressure @ 6×10-7 Torr (previously @ 5×10-6 Torr)2) 15nm Ti + 15nm Au, no vacuum break Estimated “cell resistance”: about 20-25 ohm, much lower than previous measurement. Possible reasons why lower resistance is observed:1) “Au…
Localizing Basement Membrane Production in Coculture BBB Model
Introduction The basement membrane (BM) is a critical component of a healthy blood-brain barrier (BBB) model. Further, the BM breaks down in several diseases. Both pericytes and endothelial cells can contribute to basement membrane production. This post will explore a method to localize basement membrane components – collagen type IV (Col IV), fibronectin (FN), and…
Optimization of our isolation technique using Beads
ByAslanHello Everyone, I have been working on 100 and 50 nm beads since ACNMM conference to optimize our isolation technique for exosomes and verify that our approach works. Technically, based on the discussion that we had before about Peclet Number, in order to decrease this number, I have been running all my experiments with 1…
Pressurized Gold Separations – Part 2
I continued work with a couple of new wafers and settled on one that lowered the cut-off to between 5 and 10 nm gold particles. For completeness, I will also include some of the old data that has been further analyzed. All experiments were performed with BBI stock gold solutions (0.01% w/w?) diluted 1:1 with…
