Spectrophotometer raw data
Upload just for future reference. This includes the data of KMnO4 serial dilutions absorbance and the stir cell diffusion experiment.
Similar Posts
SEM of Sheared/Non-sheared HUVECs
ByAlecMotivation and Procedure After discovering that pores on 3um LP SiO2 membranes seem to get blocked following 3 hours of shear [of HUVECs], Kilean and I decided to utilized SEM to further inspect what might be doing the occluding. HUVECs were seeded onto the membranes as usual and left to grow to confluent for 24…
Measuring changes in endothelial basement membrane integrity
Introduction Inflammatory diseases have the potential to disrupt blood vessel permeability. This has serious ramifications for the blood-brain barrier (BBB), which is the interface between the vasculature and the brain. A healthy BBB allows essential nutrients to pass through while excluding molecules that might alter brain chemistry. If inflammation were to increase the BBB’s permeability…
Effectiveness of Nanosep Omega 300K centrifugal membranes in separating IgG conjugated qdots with excess free-floating antibody
ByMarinaUsed Thermofisher SiteClick qdot antibody labeling kit 625. Began with 4.0mg/mL IgG and conjugated it with an unknown amount of qDots, methods provided by the SiteClick kit. The final stage in the manual [1] is an optional cleaning of the qDots to remove any unconjugated and free-floating qDots from the mixture using the nanosep omega…
CAD-LB: Colocalization Detection Guide
Introduction In this post I will review work I have completed to further characterize the CAD-LB platform. In this set of experiments combinations of nanoparticle species were used to define colocalization limitations. More specifically, two nanoparticle species each labeled with a unique fluorescent dye (red or blue) were used to simulate pan EV and antibody…
Rhodamine concentration curve
Lingyun and I used the stir cell and spectrometer to measure the diffusion of rhodamine across the membrane. This was the first time I was able to construct an experiment in the stir cell while keeping both membranes intact. In the past one membrane would rupture or the cell would leak too much. For this…
Cell Culture Applications
ByJimI’ve added a page on cell culture applications to the knowledge wiki. Watch this page and edit it if the topic is of interest to you. Remember the wikis provide a place to comment on the changes you make … and to compare and restore prior content … … so don’t be shy.
This belongs in a notebook. Data on the blog does not need to be polished, but it should in a form that communicates a result to others. If everyone uploaded their rawest data, the blog would be unbearable to read.