Here are some more images from my ongoing cell culture project.
My previous work and protocols: ZO-1 staining of confluent RPE cells on various MgF2 substrates
I added twice the amount of primary antibody on these devices that I usually do (1:100 dilution, instead of 1:200).
Intro Recently we have been collaborating with the Moghaddam lab from the University of Florida, which specializes in the fabrication of graphene oxide (GO) membrane, to study the use GO membrane as a toxin filter or absorbent in hemodialysis and live support devices. We are responsible for the hemocompatibility component of the study and used the following…
I tested four samples (-1,1); (-1,2); (-1,3); and (-2,4) for air permeability and burst pressure. The first three samples showed no measurable air permeability for three attempts each. The sample from position (-2,4) did have a very small air permeability of 11.2 cc/(cm2*atm). Based on the results of rhodamine diffusion it seems odd that I…
UPDATE: Figure normalized by frontal surface area. This brings the glass data in line with pnc-Si (which is similar to my more recent fluorescence data). Here is my first try at putting together all the recent adsorption gels I’ve done. This data has all been obtained using the more vigorous washing step. All densitometry performed…
As seen in prior NRG post, ozone treated samples recover over about 24 hours. However, recent experiments at Gaborski Lab show high variability when using the Corona wand to treat samples with ozone. The goal of this experiment was to determine the contact angles of PDMS samples when exposed to ozone treatment. PDMS samples were…
There is a reliability/handling issue with the single slot chips. 0.5 mm x 10 mm slots, 20 nm thick membranes with 40 nm SiN reinforcement. The membranes are easily packaged in an upper fluidic PDMS layer with inlet/outlets and two PDMS struts (on the membrane side to protect from accidental contact breakage.) The first test…
As previously stated in my initial post (found here https://trace-bmps.org/nanomembranes-in-potassium-ion-selective-electrodes), I am in the process of developing a potassium Ion Selective Electrode (ISE) Sensor using our chips. Along the way I have gathered all sorts of curious but interesting data, with observations bringing both old discoveries and new oddities to light. Outlined below are some…
