New metal coated wafer#502

Overview – The purpose of this study was to perform in vitro hemocompatibility testing (in accordance with ISO standards) on material substrates that could come in contact with blood when incorporated into the final device design of a portable hemodialysis machine. Therefore, understanding the blood response to the lift-off membrane (NPN with SU-8 grid, supported…

With changing my channel height to attempt to lower the Peclet number, one important factor that is affected is the transmembrane pressure. Decreasing the channel height will significantly increase the transmembrane pressure, which may stress the membrane and cause it to break. However, in addition to changing the channel height, I will also be changing…

Just wanted to drop this data here in case it may be useful for others in the lab. I calculated the volumetric flow rates for one of the peristaltic pumps used in the lab for the flow circuit in cell-culture. The setting # is the bar # on the peristaltic pump controller (the 10 bars…

In this experiment, I wanted to test how quickly vacuoles form in endothelial cells when cultured on pnc-Si transwells.  Here I used 30nm SC502 RTP’ed samples with the pnc-Si side down.  I seeded P9 bEnd3 cells at 50000 cells/cm2 on the pnc-Si side, allowed the cells to attach for 2 hours and then inverted the…

Matlab Generated Endothelial Cell Alignment Distribution Top and Bottom Gel No Gel Bottom Gel Run 1 Run 2 Statistical Analysis The next step in this data analysis is to perform some form of statistical analysis. Comparing real alignment data to a set of randomly generated alignments may be a good place to start. Also, comparing the frequency…

In our previous adsorption study, all of the surfaces seemed to adsorb a similar amount of protein.  We thought this might be due to ineffective washing.  In this study I tried a better washing technique: Incubate polymer membranes, pnc-Si chips, and glass in 1mg/mL BSA for 30min Rinse by vigorously swishing in 1500mL PBS for…