1-slot membrane · fixed 0.7 × 2.0 mm field · runs entirely in your browser
Counting starts automatically as soon as you drop or choose a file. The image never leaves your computer; all processing happens locally in this page.
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cells counted
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cells / mm²
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avg cell footprint (µm²)
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equiv. cell diameter (µm)
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avg dark-region area (µm²)
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dark-region size, long × short (µm)
* Assumes a confluent monolayer. Footprint and cell diameter are the field
area divided by the cell count, so they overestimate if the cells do not fill the field. The
count, density, and dark-region measurements do not rely on that assumption.
Export data
CSV contents:
Detected cells (one dot per cell)
Click an empty spot to add a missed cell, or click a dot to remove a false positive. The count and every export update live. Red dots are auto-detected; green are your additions.
This is a phase-contrast image with no nuclear stain, so the tool counts the dark
perinuclear regions as a one-per-cell proxy. The dark membrane border frame is
detected and excluded. Because the monolayer is near-confluent, tightly touching
cells can occasionally share one dot and very faint cells can be missed, both of
which make the count a slight floor. The footprint number assumes the cells fill
the stated 0.7 × 2 mm field; if the imaged area differs, every micron value
scales with it (the count does not).
