W705 performed well in terms of permeability and separation of Hybrid Silica Nanoparticles. No water passed in the wet/dry format (no matter ozone or testing configuration – centrifugal or pressure cell). Reasonably high flow rates when tested in wet/wet configuration in the centrifuge. PVP enabled flow in the pressure cell in an otherwise wet/dry configuration….
Jamie and I met with Don Black with an electron microscopist that consults for NaturalNano. In efforts to test out our TEM window grids, he gave us halloysite nanotubes. These are naturally occuring and much larger than the carbon nanotubes we normally talk about. The samples were prepared in 0.5% Triton X-100 in water and…
Here’s my ‘blog’ post using Google’s Picasa photo album: A few weeks back I received nanoparticles from Ithaca startup company called Hybrid Silica Technologies (HST). They are silica particles that are infused with fluorescent dyes that are generally similar in size and coated with a PEG layer, suspended in DI H20. I chose to work…
I obtained some perplexing results today that I have re-run multiple times. Ozone treating w504 permits the passage of water in a wet/dry format w/o PVP coating. I ran several experiments with the constant pressure device with water on the top side and dry on the bottom. Some of the membranes had once been wet/wet,…
The new bottle of PVP (polyvinylpyrrolidone) sitting on the shelf was calling my name this morning. I apologize that this post is long, but its pretty exciting – I think we have half-solved the wettability problem. According to MSDS sheets, PVP is soluble in water and alcohols. Since drying water on the membrane is a…
I treated samples from w312 using our ozone equipment. Quickly, here was the protocol:Place samples in chamberTurn on O2 and supplemental Ozone generatorAfter 3 mins, turn on Lamp in main chamber for 5 mins (Heater set to 110C)Leave samples in chamber for 15 minutes after timer turns off(O2 and supplemental Ozone should still be on)Turn…
The Ozone generator works very nicely. Dave is going to be providing an in-depth user manual for us, so I’ll be somewhat brief. In this first picture, I compare an ozone treated sample against a control. Both ‘droplets’ are 3uL of DI water. The treatment protocol:– 3 min of O2 at to fill main chamber,…
Attached is a series of images we took of two populations of neutrophils above and below the membrane.I’m including by methodology for those who are interested:One population was pre-labeled with AF488 (green) antibody against L-selectin, the other with AF546 (red). The green population was suspended in a low protein buffer (~1% serum) and a 20…
Wafer 302 appears to have no pinholes and held up pretty well in the centrifuge, up to 5psi for many hours in a wet-wet format. Here are some quick numbers on flow rate over 2 hours, 3.3psi, including radial position:1x PBS (no protein)68 uL (r=4.1)91 uL (r=4.1)99 uL (r=4.5) Using Jim’s new equation. 99uL of…
SepCon EQ reaches Equilibrium in 2 1/2 hours!w310 (no pinholes)1x PBS (no protein)2 hours @ 1000rpm (3.3psi) -> 103 uL passage (56 uL/(min-cm2-psi) )2 1/2 hours @ 1500 rpm (5 psi) -> 204 uL passage (>74 uL/(min-cm2-psi) ) In the second case, the sample visibly reached what looked like equilibrium. It was not quite exact,…
