Introduction As we’ve been trying for nanoparticle-based CAD-IA for multiple trials, we have tested various reaction routes, reaction conditions, and antibody pairs with 3 different nanoparticles. Here, I would like to take a step back to go through the CAD-IA stories based on different nanoparticles. CAD-IA based on different nanoparticles Fluorescent SA-modified magnetic particles Physical…
Introduction As what we’ve learned from the previous “Catch-and-Display Immunoassay” (CAD-IA) trials, we switched to an antibody pair from OriGene with low non-specific binding ability to each other and start to conduct ultracentrifugation for all reactive reagents for the reduction of protein aggregations. However, we found an inconsistency of co-localization percentage of immunocomplexes and beads…
Introduction The glial fibrillary acidic protein (GFAP) is an intermediate protein released by astrocytes and has value as a serum-based biomarker of neurological abnormalities including traumatic brain injury1. Inspired by the CAD-LB method for digital assessment of biomarkers on small extracellular vesicles, we sought to create a digital immunoassay for the detection of protein biomarkers….
