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AJA membrane
While Chris and I were in Boston we deposited our membrane stack on a patterned wafer. It was annealed at 1000 C and then etched. Because we didn’t have an accurate deposition rate for Silicon, we overestimated and put down 30 nm (instead of 15 nm). Still, we can see in the TEM that pores…
Designing a two-channel 6-plex μSiM platform
BypmiltonOverview The main aim of my rotation in the McGrath lab was to design a new 6-plex two-channel μSiM platform. Currently, individual two-channel μSiM platforms are used for tissue-on-a-chip experiments. Expanding the current two-channel single (1-plex) platform to a two-channel 6-plex setup will increase experiment throughput and efficiency, as well as provide a starting point…
MgF2 Cytocompatibility #4
Summary: I have some ok images (20x) of cells growing on MgF2. My controls still look poor. Something about the stain is causing the TCP + NPN controls to detach ahead of the MgF2 material, even if they aren’t fully dead yet. I used acetone fixation, which worked well for the materials on the nanomembrane, but…
BSA coating on pncSi (2D)
I coated the pncSi samples from W683 (no RTP) with Bovine Serum Albumin (BSA) in Tris-EDTA buffer. Three different concentrations were used: 1 mg/ml, 5 mg/ml and 10 mg/ml (usual range of BSA conc. used for coating Si surfaces). The samples were left in BSA solution for 45-60 mins at room temperature and almost dried,…
Co-Culture of ADSCs/HUVECs on 0.4um Translucent Membranes
ByAndreaScope: ADSCs were cultured on the bottom of the Corning 0.4um translucent membranes in DMEM+ 10% FBS+ 1% Pen-Strep. After 6 hours, the membranes were flipped over and placed into a 24 well plate. Of the four membranes with ADSCs cultured on them, 2 of them were seeded with HUVECs on the top side of…
Issues in achieving Neutrophil migration through collagen gels
For many months, Emma and I are trying to achieve neutrophil migration through endothelial cells and through the underlying collagen gel. I need to explain the details of my device first. The schematic is shown as below. I am using a 3 um porous membrane to allow neutrophils to cross the HUVECs in the collagen…