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Subscripts and Superscripts
Helpful Hint: H<sub>2</sub>O typed as text will yield H2O visually, and E = mc<sup>2</sup>O typed as text will yield E = mc2. Of course this affects line height, so choose your poison, I guess.
Blood cell trapping
I have been working on using the membranes to trap blood cells so that I can take force measurements with the AFM. It is impossible to perform AFM on blood cells resting on a coverslip because the water displaced by the tip on the approach pushes them away, so I am trying to use a…
Updated 100K microcon wet-wet permeability
ByMike HA while ago I made a post about the permeability through the 100K microcons in the wet-wet EQ format. I had run three membranes, and stopped (and restarted) the centrifugation at three separate time points to be able to calculate three permeability values for each membrane. When I did this I got strange results; the…
BSA diffusion across SC267
ByMike HLast week I made a post showing detection of detergent diffusion across membrane SC267. In the diffusion set up I had placed 6uL of a 50/50 volume ratio solution of 1.0% DDM and 1mg/mL BSA with 60uL of DI-water on the membranes backside. I allowed the setup to sit for two days and then used…
Carbonization – contact angle and discoloration
Last week, Dave carbonized some w501 samples for me so that I could look into discoloration with this new type of silicon stabilization. The carbonization recipe was: – 1 min 50:1 HF strip to form Si:H – Purge RTP with 100% Ar for 5 minutes – 10 min 95% Ar 5% C2H2 flush @ 200…
To Pulldown or Not to Pulldown, That is the Question: Testing the Utility of a Pulldown in µSiM-CA Bacteria Assays
The idea of adding a pulldown modification to our bacteria assay protocol came about after we started to believe that pore discovery was our rate limiting step of the transmission process, discussed here. Initially, it seemed to benefit our assay as it bumped our performance up significantly. Upon closer inspection we realized that we had…