Lab Floors Getting Cleaned on Feb 22
Please plan accordingly and be sure all moveable items are off the floor the night before.
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Designing a Reverse-Centrifugation Sepcon Variant (Part 1: Geometry)
We have an STTR grant funding us to revise the Sepcon plastic housing to enable us to do reverse centrifugation easier. As outlined in this previous post, we’re trying to remove unbound IgG from a solution of IgG-antibody conjugates using reverse centrifugation: The earlier work used Josh Winan’s jury-rigged PDMS-filled reverse centrifugation setup, but despite the…
Single-Well CytoVus w/ SiO2 Membrane – Comparisons
I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them. From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included: In the phase image, the pores can be seen through the cells, especially evident in…
Integrated Nano Developer Flow Rates
This experiment compares the metal developer used in Integrated Nano Technologies’ device to to the flow properties of water. Using the pressure cell at 3 psi, with no allyl treatment, for 10 minutes, and 200 microliters of liquid, I pushed water and developer through our membranes and examined the difference in flow rates. All of…
Inventory of Nanoparticles
Nanoparticles Inventory – taken by Karl on 11/2/12. All these particles can be found in the refrigerator. Manufacturer size type amount (approx) expiration date fluorescence (if applicable) BBI 5nm gold colloid 3mL Jan-10 BBI 5nm gold colloid 6mL Aug-11 BBI 10nm gold colloid 2mL Jun-11 BBI 10nm gold colloid 2mL May-09 BBI 15nm gold colloid…
Success with PDMS-Glass Bonding
ByMike HYesterday afternoon I made a new batch of 10:1 (base : curing agent) PDMS and put it in the oven at 80C for two hours to allow it to cure faster (compared to the three days it takes at room temperature). Once the PDMS was cured Henry used the UV Ozone system to again try…
Continued Reverse centrifuge tests
Our first attempt to “reverse” centrifuge materials using our standard 5-slot filters made of NPN from wafer 1070 are detailed in a previous post using GEN2 SEPCON assemblies. In this post we modify our method to more closely match the Sartorius Centrisart approach. To that end, we switch to using GEN3 SEPCONs because they are easier…