Over winter break I put together some posters to hang up in the lab. I tried to represent in an clever way a few key areas of research that NRG is interested in:
If anyone has any ideas for more, or any suggestions for captions or improvements (I’m really not a fan of “At NRG, Anything is Possible” but it was late and I was tired), I’d love to hear them.
The last time I did live imaging on the confocal microscope was in December of 2019. Then I was imaging the capture of 1.24 µm diameter beads in 1 µm slit pore membranes to visualize the difference between steric and affinity based capture. This time around, all of the materials are different, but the process…
Last week we attempted to mimic our standard CVC recipe in the AJA system (15 nm Si, 450 C deposition temp, 1000 C RTP). I should note that the protective oxide thickness in the AJA membrane was 20 nm (not 40 nm as it was in the CVC). Below are two images comparing the AJA…
These fits are done using the math outlined in this previous post. We assume an average pore size of 50 nm and an approximate number of pores in the membrane of 1E9. 20 nm particles: 100 nm particles: Next, we were curious whether we could show the transition happen from pore constriction to…
Thanks to Sidahmed Abayzeed and Emilia Moradi. Summary: I’ve started making impedance spectroscopy measurements with a potentiostat in the iBidi devices. The data is noisy, but there is a clear change in the curve characteristic as cells grow upon the nanomembrane. NPN seems to be working better than MgF2, for these measurements. I’ve measured the impedance…
This is part of our work studying how cells adhere, spread and proliferate on varying pore size/porosity SiO2 membranes. This post will be a qualitative look at how HUVEC spread over membranes by staining for actin filaments. In a future post we will quantify the degree of spreading at 1hr, 4hrs and 24 hrs. Calculation…
Introduction In our labs previously work, difficulties in growing endothelial cells (ECs) under shear flow lead to the exploration of novel membrane technologies that permit firm EC attachment, without compromising membrane permeability or leukocyte transmigration studies. We ultimately landed on the development of effectively ‘dual-scale’ membranes that contained 3 μm pores dispersed amongst an NPN…
NRG: I am the control.