Vacuole inhibition by bafilomycin A1 (trial 2)
I wanted to confirm my results from this previous post. Here, I used lower concentrations of bafilomycin (10, 25 and 50nM) in growth media. Everything else was the same (except the cells were 1 passage older).
The results are the same-lots of vacuoles in the control sample and no vacuoles at all in any of the drug-treated samples. Also, cell morphology is slightly altered in drug treated samples. I did duplicates of this experiment, but I’m only showing 1 of the samples for each concentration here. More evidence that these are vacuoles.