I set up diffusion experiments using the wafers that Tom was able to pass gold through in DI H2O using pressure. 5nm particles do not pass in 24 or 48 hours. If we have extra samples I will be testing 5nm particles in 10mM KCl.
Wet Etching of Optical Thin Films As a result of Christian’s trip and Kevin’s discussions with me, I have been wondering if we could make nanoporous MgF2 membranes for Raman spectroscopy. Part of the appeal in using our Si-based nanomembranes is that the volume they occupy in the observation space is extremely minimal. Even…
Over the last week, I’ve run a discoloration assay similar to one run a while back (Barrett’s post here). It was demonstrated in a subsequent paper (Agrawal2010) that annealing the pnc-Si films prevents discoloration of the membrane, which he tied to the nanomembrane remaining intact. I was given a few chips from the recent TEOS lot,…
Introduction This experiment is a continuation of previous work, cited below. Instead of urea spiked 1x PBS, whole heparinized bovine blood was pushed through the device over a period of four hours to then perform a urea assay on the separated and collected plasma. Please refer to the post below regarding the experimental setup that…
Thus far three HUVEC and two L13T3 growth tests have been conducted on substrates including glass,pnc-Si, tissue culture plastic, and untreated plastic. Only one test with untreated plastic has been run for each cell type. Cells on Si samples are not on the slits but rather on substrate surrounding. It seems that HUVEC proliferate more…
Previously we have studied protein adsorption to pnc-Si membranes by TEM and fluorescence. Neither of these methods will work with polymer membranes. Polymer membranes are too thick to view using TEM. Polymer membranes also autofluoresce creating a lot of noise while using our fluorescence method. Here I outline a new method of studying adsorption to…
This is just an update on the progress being made towards creating a closed flow cell by bonding PDMS to our membrane chips. For those who were not at the meeting on Tuesday we have successfully bonded PDMS to both glass cover slips as well as out membrane chips using the UV Ozone cleaner. Yesterday…
