Update of W637

We previously explored cell migration and cell-substrate interactions in the form of altered f-actin polymerization, focal adhesion formation, and fibronectin fibrillogenesis on the discontinuous substrates (porous membranes and non-fouling PEG islands). Here, we were curious about mechano-sensing on these substrates. We first investigated the f-actin polymerization and focal adhesion formation of Adipose-derived stem cells (ADSCs)…

I would like to introduce Compressive Structured Illumination Microscope (CS-SIM), which will be built in the McGrath’s Lab. CS-SIM is a novel microscopy framework, which was proposed by me. It is dedicated to alleviate some SIM problems such as motion artifacts as well as photobleaching, and also it might present further resolution improvement thanks to…

I found another old wafer # 760 and asked Josh Miller to deposit a 25-50-25 OSO stack.  He did a standard RTP of 1050 C, 60 s, 100 C/s.  (and Josh Miller did the EDP etch, so this is really all his work) I measured a porosity ~4.5 %, and avg. dia. ~40 nm.  For…

I’ve been working recently with amyloid-beta (AB) provided to me by some people in Brad Nilsson’s lab.  These experiments are related to the HMM diffusion experiments for our collaboration with Harold Smith.  Basically, AB fibrils are very long (microns) and we are looking for ways to either prevent their aggregation from smaller peptides/oligos, or to…

In this gel I evaluated the linear range of coomassie brilliant blue. From this test it looks like things are linear from .0156 to .125mg/mL.  My guess is that the protein ladder bands in the previous experiment fall within the range. I used the Bradford assay to find the total concentration of the stock protein…