HUVEC adhesion update
Couple of HUVEC adhesion trials were carried out and the results were found satisfactory as seen in the charts below.
Similar Posts
DDM diffusion through SC267
ByMike HIn a previous post I showed that a colorimetric assay using sulfuric acid and phenol can be used to establish a linear relationship between known detergent concentrations and absorbance values. This assay proves helpful in the project related to Mark Dumont’s work in that it will allow detection of small quantities of detergents that pass…
Exosome Capture from Fresh Human Plasma
ByKileanThis data is from about a month ago and it something that Jim has been asking me to put up, so here it finally is. When Tejas runs his experiments, he usually leaves me the last mL of blood that he has from his draw in case I want to do anything with it. One…
HMM vs. LMM diffusion
My last post dealing with this project was the first to definitively demonstrate HMM diffusion through pnc-Si. Unfortunately, there wasn’t a difference between the HMM filtrate signal for intact and digested (+RNAse) samples. As a reminder, HMM is a large protein multimer held together, partly, with RNA. We hope to show that HMM can not…
Endothelial cells on different width membrane windows
Along with differently sized square membranes, I also designed a wafer with different sizes of slits. On each chip, there is a 4000, 2000, 1000 and 500um long window. The thicknesses of these windows are 200, 100, 50 and 25um. I’m interested to see if there is an aspect ratio that causes endothelial cell alignment/tubulogenesis. …
ISEV-MRS 2019 Highlights
ByAslanHello Everyone, Here is a summary of my trip to Nashville to learn about extracellular vesicles in cancer and present my work on isolation of nanoparticles/EVs using ultrathin nanomembranes. Main points of the conference: This conference was about EVs in cancer, and did not include a lot about therapeutic applications of EVs. I could only…
Exploring Formlabs SLA 3D printing for cell culture applications
The initial purpose of exploring formlabs SLA 3D printer for a collaboration with Dr. Nicola Marchi (link to previous post). The purpose of the this work was gaining the ability to use a simple membrane on 3D printed platform for BBB modeling and WBC migration though endothelial (and glial cell layers) and collecting and…