The permeability data is very different for films deposited at room temeperature and 400C.
The 400C deposited wafers have much higher permeability and more consistent.
w335 – RT
w336-RT
w338 -400C
w340 – 400C
Burst Pressures
Introduction In the Glading Lab we study a rare disease called Cerebral Cavernous Malformations (CCMs). In this disease, brain capillaries develop greatly enlarged lumens and a disrupted blood-brain barrier. A genetic cause of the disease is the presence of a mutation in KRIT1 that causes loss of KRIT1 protein function in the cells. KRIT1 binds…
A bit of background: In order to quantify how permeable a tissue barrier is, we use a method known as trans epithelial electrical resistance (TEER). Cells are grown on a permeable growth substrate in a transwell format. When the cells are not confluent, there is a continuous diffusion of ions across the two sides of…
Nakul and I noted last week that the UV-ozone system was not performing. We took pictures of control and treated samples (2x) to measure contact angles on Amorphous, pnc-Si, Oxide and Nitride TEM grids. Our results were pathetic – most samples had less than a 10 degree decrease in contact angle, staying near 60 degrees….
For year one of the Trace grant, I will be focusing on the development of a standard operating procedure for the uSiM-CA. To begin this process, I am transferring all of my current operating procedures from the Andor dragonfly confocal scope to the new Etaluma microscope. The Etaluma scope will be automated, collecting images of…
Previously, I had gotten some inexplicable results showing that increasing salt concentration decreases the flow through a membrane. This makes no sense (and goes against our previous data) because we would expect electrostatic forces to counteract the flow, an effect called counterelectroosmosis by Zydney: “The situation in the presence of a net fluid flow through…
Barrett had discovered that growing endothelial cells on pnc-si or tranwells causes vacuoles to form. The vacuoles on pnc-si formed only on the free standing areas of the membrane where there are open pores. The background on pnc-si chips showed negligible vacuole formation. The density of vacuoles on pnc-si was almost two fold higher than…
