Journal Club: Preview from Sabrina Leslie – CLiC with flow

One of the issues with my microporous MgF2 cell culture experiments is the apparent multilayer of cells that form on the freestanding area; ideally we would have a nice cell monolayer for imaging. It has been unclear if this was an effect of overseeding or if cells were migrating to the freestanding area. To this…

In order from left to right: 1) t=0, huvec on pncSi, in EGM (10% FBS) 2) t=24 hrs (cells reach confluence) 3) DIC after staining 4) FITC (live cells) 5) Rhodamine (dead cells) Its good to see the cells reach confluence and the membranes intact at the same time! There is a decrease in number…

Since precision printing FITC KODE molecules into the windows of our 3 slot chips I have begun testing both the uniformity and the functionality of surface coatings of these molecules. In pursuit of coating uniformity I have tested several different methods of surface coating to determine which method provides the greatest uniformity. In this same…

Sarah and I did some more gold separations with 10, 20, 40, 50, 60, 80 and 100 nm Au particles.  We used the “clean” water purchased from the medical center.  Same as last time, we diluted the “as-bought” gold 3 parts to 1 and loaded each cell with 300 uL before pressurizing at 5 psi…

We have two cutoffs for the Millipore Biomax PES membranes: 50kD and 30kD.  I tested the “EO” rates for both of these membranes and plotted them in this figure with standard deviations (3 trials  of 50kD and 4 of 30kD). 50kD has a significantly higher rate of “electro-osmosis.”  I will try to compare these rates…