Capillary Tubing and Adapter

I reran the 100K Microcon membranes using bovine albumin. This time I ran a set of four fresh membranes with DI water for a preliminary trial. Once complete I used two of the same four membranes with a 1mg/mL solution of bovine albumin in PBS to test the effects of protein; the other two membranes…

When the HUVECs were first brought up, the first test for me to perform was to see how they reacted to CMFDA and repeated fluorescent excitations.  Based on the implications from previous research (summarized here), there is the possibility of harm or phototoxicity from the dye.  Since HUVECs are more valuable and ‘fragile’ than either…

I’ve been doing some preliminary studies with HUVEC in commercial transwells and on pnc-Si in the Sepcon/transwell hybrid format.  The thinness of pnc-Si will enable better microscopic examination of cells than other membrane systems (if pnc-Si is flat).  I thought it would be interesting to compare commercial transwells to the pnc-Si “hybrid transwell” format in 24-well plates via…

As I am hoping to promote tighter junctions between endothelial cells in my device, I am trying to create an environment that produces shear stress on the endothelial cells. Shear stress up-regulates the pathways that promote tight junctions (Tzima et al 2005, Dejanna et al 2004). The value of the quantity is somewhere around 6…

EDIT – 2/10/14 – Adding 1 mM phosphate buffer fixes the problems outlined in this post. I’ve been having some trouble taking consistent pH measurements with the new pH probe. Prior to Thanksgiving I made a spectrum of 10 mM KCl buffers (at 4, 6, 7, 8, and 10 pH units) for use in my…