Porous TEM windows fabrication using CsCl self-assembly

I was motivated to scan our membrane edges after looking at this image from the paper Maryna presented last week: I found a couple of pnc-Si samples with membranes still attached to the edges.  I also found one sample with a membrane split almost down the middle.  I coated the samples with 3nm of Au…

The idea of this project started when we were writing a mini-review about cell-substrate interaction. Although most of studies of cellular responses to porous membranes have pointed towards a multiphasic response with the same trend of changes with respect to the pore sizes, we also found some variation in the results. We suggested that some…

I designed new masks with different sized squares and slits in order to study how the aspect ratio of free-standing membranes would affect endothelial cells.  In this 1st experiment, I seeded bEnd at 50000 cells/cm2 and let them grow for 4 days.  I then stained the cells with Live/Dead and overlaid the phase channel in…

The question arose last week about the affects of transport with use of the shaker in the Tecan, which led to a discussion about the current construction of the sepcon. The sepcon layout currently is as follows: The o-ring is 1 mm in diameter. Is this difference between the membrane and the receiver fluid dead…

For year one of the Trace grant, I will be focusing on the development of a standard operating procedure for the uSiM-CA. To begin this process, I am transferring all of my current operating procedures from the Andor dragonfly confocal scope to the new Etaluma microscope. The Etaluma scope will be automated, collecting images of…

The basics of this device are… Blood flows through a filter made of zeolite containing nanofibers. While the blood flows transversely through the mesh, creatinine can diffuse through the mesh where zeolites stand by to grab them via size selecting capture. Their experiments were done with 190 µM creatinine solution. MW of creatinine is 113.1179…