In this week’s meeting there was a request to have Maryna and I make our BP and AP data available online. While Maryna is at the Gordon Conference I thought I would upload what I have. The attached spreadsheet contains two tabs, one for AP and BP. Wafer number and position of each test sample are shown, along with other information. Enjoy!
I update this post weekly to reflect newly tested wafers.
With the installation of the new STED (STimulated Emission Depletion) system in the medical center, I have finally been able to work on imaging individual exosomes (that we know are exosomes because they are labeled for exosomal markers) that have been captured on our membranes. Now, I won’t go fully into the details of how…
Here are some other in-situ heating videos I made recently. First one is NSN30730, which means the Si is 7nm and Si3N4 is 30nm. The speed of the video is 10 time faster than the real time. The first figure is the heating illustration and then comes with the video. The next is sample NSN301030….
One of the many contributions Alec S. made to our group is to help us appreciate the importance of the apicobasal (A/B) polarity of endothelial cells. The effects of A/B polarity were demonstrated in his paper: Integrative Biology (2020), 12(11),275–289 where he showed that stimulation of ECs with TNFα from the abluminal chamber caused an…
Previously we have worried about the validity of our adsorption data considering we have used it to test both sides of anisotropic membranes that have cutoffs below the size of albumin. Today we came up with the idea that albumin can be used as a probe for adsorption, not as simply a model protein in…
It’s been a while since I’ve done long-term BBB co-culture experiments on transwells but I recently completed a study. I was particularly interested to determine whether I could reproduce this spike in TEER that I measured in 1 previous experiment. I also needed repeats anyway. I followed the normal procedure – seeded P11 bEnd3 on…
I talked with Jim last Friday about a second possible dialysis system using PDMS bound to a glass cover slip. I made a batch of PDMS and let it cure at room temperature over the past few days. Jim had suggested I try bonding the PDMS to a glass cover slip using the UV/Ozone system…
Thanks Karl. I will put these through a JMP analysis this weekend.