Update of W637

Molar flow thought pores with different diameters under 1 psi. First, I calculated molar flow based on porosity histograms extracted from TEM images. And then compared it with the actual experimental data. Here are few examples of this. molar%20flow.ppt

So, I have been working with a 4-probe TEER measurement system to record the changes in endothelial permeability during neutrophil extravasation and other biochemical assays such as thrombin treatment and TNF stimulation. 4-probe systems are sensitive because they don’t suffer from the contact impedances and measure only the potential drop across the sample under consideration. See…

The 20nm deposited polymer film permeability is approximately 1e6 barrers, while bulk polyimide permeability is less then 100. So the experimental value is too high. There are two possibilities to explain it: First, the pores are partially open and 20 nm thick layer is too thin to cover big pores. I will try AFM this…

Introduction The work in this post explores the hypothesis that durotaxis affects the invasion of S. aureus into the osteocyte lacuna-canicular network (OLCN). To address this hypothesis, we modified the µSiM to enable the independent tuning of pore stiffness, ranging from those of soft-tissues to that of silicon nitride (~10X stiffer than bone). More background…

I think the consistently low flow rates are a real bottleneck for all our fluid based experiments and must be overcome. On the production side, we are really pushing the limits of our capabilities to produce the current membrane geometry, which I consider to be crazy big at 0.1 x 2 mm!! Considering the 15…