I would like to share my successful immunofluorescence stainings of adhesion molecules and junctional proteins of hiPSC-derived brain microvascular endothelial cell (BMEC)-like cells on nanomembranes. The cells were differentiated by the extended endothelial cell culture method (EECM). You can find…

Below is a video protocol for wetting a sepcon device. There is no audio but amounts are detailed.

The following procedure is a first draft of the procedure used by Jim and I in an attempt to label BSA with TRITC. This is likely to be updated with photos of the procedure in the future. Prepare reaction buffer…

Following is a written protocol for SEPCON assembly.  (Here is a link to a video demonstration) Materials required: wafer tweezers, small sample tweezers (preferably plastic), soft foam board from craft store, wafer of sepcon chips, razor blade, plastic enclosure to…

SEPCON assembly

I got some experiences in dealing with cells. This is for your reference.  Aseptic Technique and Good Cell Culture Practice  Sanitize the cabinet using 70% ethanol before commencing work.  Sanitize gloves by washing them in 70% ethanol  Put all materials…

I’ve been working over the holiday on trying to make Ag/AgCl electrodes.  Here’s the method I’ve come up with so far: 1. 99.99% silver wire is used as the anode (+) and platinum as the cathod (-). 2. Silver wire…