Introduction The modular μSiM is based on a 96-well plate format with a 100 μl open well. While these devices have proven extremely useful for various barrier models including the blood-brain barrier1 and the tendon-vascular interface2, there is a need…

Introduction A recent collaborative publication from RIT developed a modular µSiM to address the lack of physiological fluid flow in conventional open-well devices (Figure 1). The magnetic latching assembly of this design allows for quick configuration into a fluidic setup.…

One of the more useful formulas for our materials is Equation 1 in Chung et al., 2014 Lab on a Chip 14:2456-68 Assuming uniformly distributed pores in a planar (i.e. ultrathin) membrane, the relationship between pore radius r, porosity p,…

1 m^3 = 1000 L 1 cm^3 = mL 1 mm^3 = uL 1 um^3 = fL 100 µm x 100 µm x 100 µm = 1 nL 100,000 nm^3 (50 nm tall cylinder with 50 nm diameter) = 0.1…

Wright, E., Miller, J.J, Csorda, M.. Gosselin, A.R., Carter, J.A., McGrath, J.L., Latulippe, D.R., Roussie, J.A. (2020) Development of Isoporous Microslit Silicon Nitride Membranes for Sterile Filtration Applications Biotechnology & Bioengineering https://doi.org/10.1002/bit.27240 DesOrmeaux, J. P., Winans, J. D., Wayson, S.…

Because of the non-linear dependence of volumetric flow rate on pore size … where t is the membrane thickness and r is the pore radius, large pores contribute disproportionately to the total flow through a membrane than small pores. The…

This figure appears in our 2016 JMS paper and describes the clogging behavior of NPN with a 37 nm average pore size. The y-axis is the volume of filtrate after 1 min of forward centrifugation at 690g (3000 RPM). The…

When we coat surfaces or particles with proteins we often wonder, ‘how long until the coating is done? ‘ In this 2009 paper we addressed the question directly. We soaked 100 nm polystyrene particles in 10% serum (about 6 mg/ml…

Knowledge of the particle number is critical in many of our applications. In trying to rationalize membrane ‘clogging’ by particles for example, it is wise to compare the particle density to pore density at the membrane. When conjugating protein to…

Darcy’s Law relates the volumetric flow rate Q through a porous media in response to a pressure gradient . … Where is the intrinsic permeability of the media, is the fluid viscosity, L is the length of the media that…