TRITC tagging of Fetal Bovine Serum, TECAN v ImageJ measurements
pnc-Si chips prewetted with PBS then incubated with fetal bovine serum (unknown molarity) for 2 hours under refrigerated conditions. Thoroughly rinsed with reaction buffer (perfused 5 times). TRITC (2 mg/mL) added to dry chips and incubated at room temperature for one hour. Then rinsed once with reaction buffer then rinsed thoroughly with distilled de-ionized (DDI)…