I am trying to grow HUVECs under flow in my device to establish a tight and confluent monolayer for TEER analysis. My device schematic looks like this and the whole setup is as below I tried to use the NPN chip in sepcon format so that I don’t have to be restricted by number of…
The purpose of the fluidics in our hemodialysis system is to move the sample to and the retentate away from the membranes. The dialysate (permeate, filtrate) will be handled passively in the initial designs and therefore do not require fluidic manipulation. The parameters influencing the movement of the sample past the membranes include channel geometry…
Hi all, After nearly a month of troubleshooting the fabrication of Whitesides Chamber we finally have the capability to generate a steady and linear gradient of molecules. In the presented case, we have generated a gradient of rhodamine 6G (R6G), which is a fluorophore that allows us to visualize and confirm the existence of the…
The pump’s data sheet (located here) describes the pumps materials as: polyphenylene sulphone (PPSU) polyimide (PI) nitrile butadiene rubber (NBR) It also says that the acceptable particle tolerance is < 50 μm. This is the info we will need to determine the types of particles we can use with an in-line flow system.
Based on the results obtained yesterday with the closed flow cell we reran another trial today. The closed flow system we tested today was bound using UV Ozone treatment, surprisingly this system created a relatively strong bond despite our resent results. Once the UV Ozone treatment was complete and the system had bound vacuum grease…
Following the advice of Dave and Chris I ran a UV Ozone bonding trial yesterday with the stage temperature set to 100C (instead of the usual 25C). The idea behind the elevated stage temperature is to drive off any water vapor that might be within the UV Ozone chamber during the treatment process. At the…
