This post is a brief update on the previous post of HUVEC migration on 0.5 μm porous substrates. Brief Recap We compared cell migration on nonporous (np), 0.5 μm low porosity, and 0.5 μm high porosity SiO2 membranes. Low and high porosity refer to a center-to-center distance of two or three pore diameters, respectively. For…
Intro: Hi all, as we investigate the formation of HUVEC focal adhesions on porous SiO2 membrane , we also looked at fibronectin fibriollogenesis. Interestingly, it appears that after 24 hr (since the initial seeding), the fibronectin fibrils formed by HUVEC vary depending on the pore characteristics of the SiO2 membrane. In this case, we looked at…
Goal: study HUVEC migration on different porous substrates. HP = high porosity 0.5 μm SiO2 membrane LP = low porosity 0.5 μm SiO2 membrane NP = non-porous SiO2 membrane TCP = tissue culture polystyrene (~3 GPa [ref 1, 2]) P184 = sylgard 184 (a commonly used PDMS; Young’s modulus = 1.74-1.84 MPa [ref 3]) P527 = sylgard 527…
Purpose: Study the hemocompatibility of different substrates using platelet activation as the indicator. Extraction of platelet rich plasma (PRP): PRP were prepared from whole blood following the standard protocol outlined by Abcam (http://www.abcam.com/protocols/isolation-of-human-platelets-from-whole-blood). To describe briefly, 9 mL whole blood was centrifuged at 200g for 20 minutes in a 15 mL Falcon tube to obtain…
This post is an update of the TAT ELISA study. Details of which can be found in the prior post by Keisha and I: https://trace-bmps.org/blog/data/2016/02/18/hemocompatibility-studies This is the most up-to-date data: The error bar represents the standard error of mean. n = 3 for each group NOTE: We used serum as the positive control…. serum is…
Purpose: Study the hemocompatibility of different substrates using the concentration of C3a as the indicator. Background: C3a is a protein produced during immune response. Directly from Wikipedia: The classical pathway of complement activation is initiated when the C1 complex, made up of C1r and C1s serine proteases, recognizes the Fc region of IgM or IgG…
Hi All, Presented in this post is the following paper, titled: “Detection and isolation of circulating exosomes and microvesicles for cancer monitoring and diagnostics using micro-/nano-based devices” [REVIEW] exosome handling in micro and nano devices Keynote presentation: [PRSENTATION] NRG (2015-11-16) exosomes
Xunzhi from the Shestopalov lab gave us a brief presentation on how XPS is used to assess surface modification of the nanoporous nitiride silicon (NPN) membrane chip. Power Point Presentation: surface assessment via XPS
The presented post is somewhat of a follow-up of this previous post, in which we studied the adsorption of proteins on silanized NPN chips. In the presented work, we study the adsorption of fluorescent proteins (BSA and IgG) on both the untreated and the PEGylated nanoporous nitride membrane (NPN) chips using epifluorescence as the imaging…
An application of nanothin and highly porous (NTHP) membranes for mesenchymal stem cell (MSC) differentiation in a co-culture setting. The highlight of this article is that the NTHP membrane allows direct contact of the MSC to cardial myoblast (H9C2) while still maintaining the separation of the different cell types. This ultimately leads to higher degree…
