We borrowed a method first published by Jahn et alii about 7 years ago, in which lipid dissolved in ethanol are streamed through a central channel and compressed by 2 streams of buffer (we used PBS). This hydrodynamic focusing results in the generation of liposomes at the interface of the miscible liquids. This results in…
In this post I address every objective we started with and summarize our results: a) Building of research capacity at Fisher. —–> We would like to to produce our lipid-based nano particles in a cheaper and more reproducible way. For the last 4 weeks Ryan has been producing phosphatidylcholine liposomes. Depending on the rate of flow, we…
In our previous post I relate how our first experience with the membranes encountered some difficulties. These were mostly of two kinds: clogging or braking of the membrane and somewhat arbitrary sizing of the particles. We think we have fixed both. In our latest experiments we have used manually-extruded particles. These are obtained after the…
Josh has just started teaching us how to play with these; some early findings: – So far, standard centrifugation results mostly on the caking of lipids that clogs the membranes. – Use of the pressure cell has shown some promise, we have passed 120nm lipid-nps through 400nm pores @10PSI. The result? 70nm particles… – Reverse…
We still have plenty of things to do, but after five weeks working at the UR, we have come a long way: – Chip production for instructional use: done – Design and production of SU8 mold: done – Protocols for (@Fisher) manufacture of micro and milli (silhouette) fluidic devices: done – Construction and testing of chips…
MAKING CHIPS We now have our own SU-8 mold (with the help of Brian & Alex at URnano) and have started producing chips (see below) with our channel designs. Together with the chips produced last week, we have accomplish one of our objectives: To build a collection of chips for classroom use. With the acquisition…
HI everyone, We continued working with Henry and Greg, gaining expertise on building chips and print-cutting channels. Right now we have a bunch of chips made, some have been tested, and we have completed one of our objectives : To produce chips for teaching. We also had our mask made and we will be…
With the help of Henry and Greg, the Fisher team has been busy learning the basics. John and Ryan have learned some PDMS microfluidics-making and will have their first chips ready on Tuesday. We have also been playing with the silhouette printer, studying our options for producing some simple devices. Finally, we produced some chip designs to…
The overarching goal of this collaboration is to expose students and faculty at primarily-undegraduate institutions to the technology & expertise available at the University of Rochester. We would like to make this kind of experience a recurring theme at URNano, and we expect to lay the foundations of such a program during the Summer of…
Hello NRG! Thanks to Jim McGrath and NSF, St. John Fisher College students and myself will have the opportunity to conduct research at URNano and the McGrath Lab this summer. I am an Assistant Professor at Fisher in the Biology Department, and my background is in cellular immunology, with a focus on sterile inflammation. Why…
