Metallized Membranes

In my last post on this subject, I showed that cutting off permeability of pnc-Si chips inhibits vacuole formation in endothelial cells.  To do this, I seeded cells on the membrane side, allowed them to attach, inverted them onto a media bubble contained within a cloning ring, wet the well-side with media and then placed…

I’ve redesigned the co-culture devices described in an earlier post to allow for media isolation as well as to alleviate concerns regarding bubble trapping. The new design is pictured below: The large cube of silgard builds a cylindrical reservoir for media and can support upwards of 100 uL of fluid (equivalent to other co-culture devices…

Demos of the Molecular Devices M2e and Tecan Infinite M200 are done. Erik from Tecan left us the M200 to use until October 6th.  Please come try out your assays on it.  Or check sensitivities.  Jess or I could probably steer you in the right direction as far as the software is concerned. My impressions…

One of the other suggestions from Angela Glading at lab meeting a while ago was to quantify the vacuoles on different culture areas.  So, I selected images from 3 different experiments (all at day14 of growth) and counted the number of cells which expressed vacuoles on different areas.  I then used my mask code in…

Here are the results of a protein ladder separation with w677.  If you recall, 677 is the 200um wafer that did not fit into the SepCons but had very few pinholes. S = Standards Ladder, C = Protein solution that never came in contact with membrane, R = Retentate, F = Filtrate Myosin (Band at…