Alec’s Proposal Exam Documents

In this post I will explain how I tried to use the 1D diffusion model to check the results we had in the Nature paper.  Unfortunately I didn’t get the same results in the simulation and I present some ideas for the discrepancy. In the Nature paper we made simple small volume diffusion chambers and…

The human IgG did not seem to diffuse through the membrane the first time we conducted a diffusion experiment. Barrett believed that the lack of diffusion was most likely due to protein aggregates blocking the pores. So, the human IgG was filtered, and this post is the result of a second round of diffusion experiments….

Those with a great memory will remember that I’ve been struggling (1, 2, 3) to identify adherens and tight junction proteins (i.e., cadherins and ZO-1) in bEnd3 endothelial cells.  Since I’ve recently moved over to investigate air-blood barrier co-culture models, I decided to see if my epithelial cells (RLE-6TN) express cadherins (they should since these…

Here are the first experiments I have performed with the SepCon slots, under the guidance of Jamie and Kilean. The goal of these experiments is to prove that goat anti-human beads (6.8 um diameter) can be combined with a low concentration of Human IgG (1 mg/mL) which will cause aggregation, and therefore accumulate on the…

The first DNA extraction was done over 150 years ago in 18691. Since then, the process has been refined and expanded to include multiple physical and or chemical methods. For the waste water epidemiology project (described here), we will be focusing on DNA extraction via silica adsorption without the use of chaotropic agents. The mechanism…