Hi All,
These are documents associated with my proposal: Silicon Nanomembranes for Rapid Liquid Biopsies from Whole Blood and Urine.
Proposal: Kilean_Lucas_PhD_Thesis_Proposal_Reduced File
Presentation: Proposal Presentation_03
I used COMSOL for finite element analysis (FEA) modeling of EOF and DEP at a microporous membrane in a PDMS microfluidic channel. Effects of two parameters were determined: Membrane thickness, and spacing between pores. The modeled membranes had a 25×25 um surface, and a thickness of either 100 nm or 500 nm. Pore diameter was…
Papers: Multi-Nanopore Force Spectroscopy for DNA Analysis Control of DNA Capture by Nanofluidic Transistors 2013 SingleMoleculeReview
The fabrication of nano pocket membrane to capture and release the EV faced challenges during the etching step. There was a suggestion after the last NRG post to use RIE-2000 located in the UR. This post shows the update on the methodology of creating the nano pocket membranes and the preliminary results after using the…
This is simply the latest repeat of a blood-brain barrier co-culture on PET and pnc-Si transwells. These are P17 bEnd and P24 NG10815, each seeded at 50000 cells/cm2. As usual, I seeded bEnd on the pnc-Si side, upside down, and NG10815 on the well side, facing up. I took TEER measurements every other day for…
I made a post yesterday showing that in 20mM HEPES, pH7.5, +10mM NaCl solution equivalent amounts of DDM were diffusing through the “treated” and “untreated” SC256 samples. Additionally it appeared that minimal amounts of BSA were diffusing across either sample. I set up another diffusion experiment and let it sit overnight. DDM analysis: As observed…
Hey folks, Science Saturday is this Saturday and Sunday at the Rochester Science Center. We need volunteers to man our tables from 12-4pm on both Saturday and Sunday. Please indicate in the comments of this post if you can volunteer, and when you can volunteer. The tentative schedule is as follows: 12-1 Saturday – Greg,…
