1) Oxidation:
- UV Ozone treatment: 5 minutes with secondary chamber ‘ON’ followed by ‘trigger’ which keeps the primary chamber ‘ON’ for another 5 minutes. After next 5 minutes, switch ‘OFF’ the secondary chamber and then the whole system. (Total=15mins.)
- Plasma Oxidation.
2) Mix 5ml dH2O with 4.8ml of MeOH and mix it well after covering it. Add 0.2ml of APTES (3-Aminopropyltriethoxysilane) (Sigma-Aldrich, CAT No. A3648/440140) to this using a syringe followed by 40ul of glacial acetic acid. Stir it well and let it stand for ~10 minutes. Keep the solution covered at all times. (make sure that the solution is clear)
3) Get the UV Ozone treated samples and dip them in the above solution (taken in a petridish) for 15 minutes. Use vacuum grease to keep the samples lifted above the floor so that both sides are treated.
4) Take out the samples carefully and rinse in dH2O gently. Keep IPA dripping through the tweezers as you take the sample out from water and then dip it in Pentane for roughly 10 secs. Use Nitrogen gas in a beaker to dry the sample.
5) After drying, bake the samples in Oven @ 75C for 30 minutes or in UV Heat chamber @ 100C for 20 minutes.
Amino-silanized samples are ready for use.
Sources:
Methods section of our Nature Paper.
Ouyang, Striemer, Fauchet. 2006. Appl. Phys. Lett. 88:163108.
Bioconjugate Techniques. Greg T. Hermanson.
Protocol from DeLouise Lab.
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