Coulter Project Timeline

In my 1st post detailing glial cells on pnc-Si transwells, I saw lots of cell clumps above the membrane window (see here).  Since these cells were grown in the wells, I thought they could be piling on top of each other in the wells and getting trapped.  Here, I decided to flip the sample so…

I got back on the AFM today to rescan the wafers from yesterday and too scan a wafer that Dave had given me a few weeks ago.  The 2 panels are from the height channel, I used the 240 tip (yesterday I used the 160 tip).  The top panel is the wafer from Dave and…

I’ve taken fluorescence measurements with the fluorescence microscope on PEGylated and unPEGylated glass coverslips and SiN chips. Six coverslips and six chips were prepared. Samples were treated exactly the same, save for the fact that half the samples had PEG Silane in their treatment and half did not (though they were still bathed in toluene…

Introduction After completing my experiments on burst pressure of HD membranes, I started the experiments on finding the burst pressure of 3-channel nitrite chips by using the airgas cylinder. This experiment is to investigate if soaking in viscous fluid will significantly weaken the membrane and lead to burst. The chip was placed on the center…

The following is a public service announcement: I’ve just aliquoted about 40mL of fluorescent BSA and placed it in the freezer. Each aliquot contains roughly 1mL of 6mg/mL TRITC-conjugate BSA, so there’s plenty of them. If anyone needs fluorescent BSA for anything, feel free to take as much as you need from the box labeled “TRITC-conjugate BSA”…

In my previous post on applying voltage to a silver-coated NPN chip, I put up a video which, if you squinted and were willing to be charitable, showed that I could modify the EO pumping behavior of the chip by applying 2 V to the membrane. I repeated the experiment, and I think I can…